1| Prepare a 40-ml 4.5% native acrylamide gel (using 1 to 1.5-mm spacers): Prerun the 'gel for 2 h at 10 mA. Large protein complexes greater than 1 MDa in mass are difficult to resolve and migrate ...

While researchers have long relied on electrophoretic mobility-shift assays (called gel shifts) to identify which transcription factors bind to DNA samples, the method can be time-consuming and ...

Researchers interested in evaluating the interaction of transcription factors with their target sequences commonly employ the electrophoretic mobility shift assay, more commonly known as the ...

Or maybe not. See how researchers used DNA footprinting and gel shift assays to prove this idea. A typical footprinting experiment involves radioactive labeling of the DNA region of interest ...