The pI markers and protein of interest migrate through the capillary until they reach a pH value where their net charge is zero—this is their isoelectric point. The iCE3 then uses whole-column ...

Table 2 summarizes the conditions used for a linear additive titration. A sample of magnesium hydroxide was titrated with FeCl3 to try and optimize dispersion conditions. A stock FeCl 3 solution of ...

We have an Agilent Offgel fractionator capable of separating either intact proteins or protease fragments into as many as 24 fractions based on isoelectric point. After a quick clean-up step, the ...

In this method, first the protein is passed in a narrow path of isoelectric targeting gel. Based on the isoelectric point the protein can be separated.

Writing in the Journal of Agricultural and Food Chemistry, the Finnish researchers report that modifying the isoelectric point of the protein could extend the range of products the ingredient could be ...

Conclusions The isoelectric point of two emulsion samples was analyzed by determining zeta potential as a function of pH using a HORIBA SZ-100 DLS. The reproducibility of sample B was then studied and ...

American Journal of Botany, Vol. 13, No. 10 (Dec., 1926), pp. 743-753 (11 pages) 1. Use was made of the method of determining isoelectric points for plant tissue which consists in placing pieces of ...

When protein is in its isoelectric point, which is at about pH 5.1, it does not bind water very well. However, when meat protein is charged, it attracts water and binds it.

American Journal of Botany, Vol. 80, No. 10 (Oct., 1993), pp. 1155-1161 (7 pages) Antiserum was raised in rabbits against a lily (Lilium longiflorum) anther-specific glycoprotein (LLA-75). LLA-75 ...

This technique was first proposed in 1979 and was originally called protein blotting; at the time, existing techniques for purifying proteins associated with DNA had to be combined to produce results.