The possibility that a contaminating protein with SOD activity might have been copurified with germin was excluded by electroblotting of the native germin band and N-terminal sequencing.

Following electroblotting, the membranes were blocked using 5% nonfat milk in TBST overnight at 4°C. After 30 minutes of washing in TBST, the membranes were probed with one of the following primary ...

Target protein samples were separated by 10% SDS-PAGE and transferred by electroblotting to polyvinylidene difluoride membranes (Millipore, MA, United States). After blocking with 5% skim milk for 2 h ...

TTN (titin) is the third myofilament type of the cardiac sarcomere and performs important functions that include generating passive tension. Changes in TTN expression are associated with cardiac ...