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Several isoforms of superoxide dismutase (SOD) with a high isoelectric point (pI) have been identified by isoelectric focusing chromatography in protein extracts from Scots pine (Pinus sylvestris) ...
In this method, first the protein is passed in a narrow path of isoelectric targeting gel. Based on the isoelectric point the protein can be separated.
The pI markers and protein of interest migrate through the capillary until they reach a pH value where their net charge is zero—this is their isoelectric point. The iCE3 then uses whole-column ...
We have an Agilent Offgel fractionator capable of separating either intact proteins or protease fragments into as many as 24 fractions based on isoelectric point. After a quick clean-up step, the ...
American Journal of Botany, Vol. 13, No. 10 (Dec., 1926), pp. 743-753 (11 pages) 1. Use was made of the method of determining isoelectric points for plant tissue which consists in placing pieces of ...
Table 2 summarizes the conditions used for a linear additive titration. A sample of magnesium hydroxide was titrated with FeCl3 to try and optimize dispersion conditions. A stock FeCl 3 solution of ...
Writing in the Journal of Agricultural and Food Chemistry, the Finnish researchers report that modifying the isoelectric point of the protein could extend the range of products the ingredient could be ...
Conclusions The isoelectric point of two emulsion samples was analyzed by determining zeta potential as a function of pH using a HORIBA SZ-100 DLS. The reproducibility of sample B was then studied and ...
When protein is in its isoelectric point, which is at about pH 5.1, it does not bind water very well. However, when meat protein is charged, it attracts water and binds it.
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