Simultaneously, formalin-fixed paraffin-embedded (FFPE) AT samples were subjected to 5 μm sectioning, 4',6-diamidino-2-phenylindole (DAPI) staining, and in situ hybridization assays, allowing ...

Green: Viability of live muscle tissue (Calcein AM staining, 4×). Red: Sarcomere striation formation within myotubes (α-Actinin staining, 20×). Blue: Muscle cell nuclei within myotubes (DAPI ...

In vitro phage-lysed bacteria provide partial protection, suggesting a combination of phage-induced lytic activity and immune stimulation by phage treatment is responsible for the effect. These ...

Markers included CD4, CD8, CD20, FoxP3, PanCK, and CCR8, alongside DAPI staining to assess immune contexture (infiltrated, excluded, desert), TLS status, and CCR8/FOXP3 double-positive Tregs. Clinical ...

Phage infection was restored in the complemented strains (Figure 6), as evidenced by the appearance of lysis zones, confirming that LPS and OmpC serve as the receptors for LEC2 and LEC10, respectively ...

Reserpine, previously identified through high-throughput screening in CEP290 retinal degeneration models, enhances photoreceptor survival in P23H rhodopsin retinal degeneration models, particularly in ...

The phage lysate was allowed to settle for 1 min at room temperature and then dried using 3-M filter paper. Negative staining was performed with 1% (w/v) uranyl acetate for 30 s, after which the ...

After 5 min, excess phage suspension was removed using Whatman cellulose filter papers and stained with 2% of freshly prepared uranyl acetate solution for 2 min. Excess stain was removed, and grids ...