EurekAlert!4d
Mayo Clinic researchers identify a measurable genetic mutation as a significant predictor of metastasis and survival in pancreatic cancer
A new study by Mayo Clinic Comprehensive Cancer Center researchers found that the presence of a specific genetic mutation — KRAS circulating tumor DNA (ctDNA) — strongly indicates a higher risk of ...
eLife5d
Pu.1/Spi1 dosage controls the turnover and maintenance of microglia in zebrafish and mammals
Microglia are brain-resident macrophages playing pivotal roles in CNS development and homeostasis. Yet, the cellular and molecular basis governing microglia maintenance ...
eLife22d
Adventitial fibroblasts direct smooth muscle cell-state transition in pulmonary vascular disease
B) Dimethylmethylene blue (DMMB) assay for quantification of glycosaminoglycan content ... H) Representative immunofluorescent localization of apoptotic cells (TUNEL marker). PASMC (ACTA2 marker) and ...
Frontiers1mon
The correlation between sperm DNA methylation and DNA damage: a comparison of comet and TUNEL
Although the comet and TUNEL assay are both used in reproductive research to assess levels of DNA damage in sperm, this project has concluded their link to epigenetic markers showcase very different ...
gut.bmj2mon
Targeting MXD1 sensitises pancreatic cancer to trametinib
We also applied single-cell RNA sequencing, Assay for Transposase-accessible Chromatin with sequencing and Cleavage Under Targets and Tagmentation sequencing to explore the relevant molecular ...
PNAS2mon
Precision targeting tumor cells using cancer-specific InDel mutations with CRISPR-Cas9
A total of 3 d post-transfection, cell viability was measured using the CellTiter-Glo Luminescent Cell Viability Assay (cat no. G7570 ... from Addgene no. 46914), replacing the mCherry marker gene ...
Frontiers7y
Non-Visual Photopigments Effects of Constant Light-Emitting Diode Light Exposure on the Inner Retina of Wistar Rats
The terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed following the procedures ... The proteins (20 µg) and molecular weight marker [5 µl ECL Rainbow Marker-Full ...