Immunohistochemistry is a form of immunostaining. It involves the process of selectively identifying antigens in cells and tissue, by exploiting the principle of antibodies binding specifically to antigens in biological tissues. Albert Hewett Coons, Ernest Berliner, Norman Jones and Hugh J Creech was the first to develop immunofluorescence in 1941.

Immunohistochemistry (IHC) combines anatomical, immunological and biochemical techniques to image discrete components in tissues by using appropriately-labeled antibodies to bind specifically to their target antigens in situ.

Jun 20, 2023 · Immunohistochemistry (IHC) allows pathologists to achieve something remarkable. It enables them to see signs of disease invisible to the naked eye. IHC uses your body’s powerful fighters, antibodies, to expose harmful, microscopic substances that cause disease.

Sep 25, 2022 · Learn how IHC testing works to help diagnose cancer more precisely, what to expect, the benefits and risks. Immunohistochemistry is a laboratory test. We're available 24/7.

Immunohistochemistry (IHC) is a widely used ancillary testing method in anatomic surgical pathology for cell classification and diagnosis and utilizes antibodies targeted against certain antigens in specific tissues and cells to facilitate determination of cell type and organ of origin.

Apr 3, 2023 · Immunohistochemistry (IHC) is a widely used staining technique that allows doctors to detect certain cancers and infectious diseases more easily. Since its introduction in 1941, scientists have...

Immunohistochemistry (or IHC) is an antibody-based technique used to characterize protein expression in tissue whose structure and organization has been preserved. The first reported use of IHC was in 1942 by Coons et al., who reported the development of a fluorescently-linked antibody to visualize pneumococcal bacteria.

Feb 5, 2025 · Immunohistochemistry (IHC) is a special test used by pathologists to detect specific molecules on cells. It is widely used in clinical oncology for categorizing solid cancer tumors and blood cancers, including leukemias and lymphomas.

Nov 15, 2024 · For researchers seeking to validate IHC assays, navigating the regulatory landscape is often complex. This article discusses critical aspects of assay development, regulatory pathways, and practical steps for successful clinical trials and assay commercialization.

Immunohistochemistry (IHC) is an ideal technique to choose when experimental aims revolve around target co-localization with other proteins of interest, subcellular target localization, and expression profiles in different cell-cycle or cell-type subpopulations.