The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay detects DNA breakage by labeling the free 3ʹ-hydroxyl termini. Given that genomic DNA …

The TUNEL assay (Terminal deoxynucleotidyl transferase dUTP nick end labeling) is a widely used technique in Cell Biology to detect DNA fragmentation resulting from apoptosis. It …

TUNEL is a method for detecting apoptotic DNA fragmentation, widely used to identify and quantify apoptotic cells, or to detect excessive DNA breakage in individual cells. [3] The assay …

Effective methods currently exist to evaluate the diverse effects of genotoxic agents and the underlying molecular mechanisms that repair DNA lesions. The current study provides an …

Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late …

TUNEL, i.e., terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling, has become a widely used staining method to assist in detection of apoptotic cells in tissue sections.

An assay that relies on detection of DNA strand breaks (DSBs) in situ by labeling them with fluorochromes has been developed to identify and quantify apoptotic cells by fluorescence …

This review outlines the general method for in situ TUNEL staining of cultured cells and tissue sections, and highlights recent improvements in the technique and limitations of the assay.

Two parallel staining techniques have been developed: the in situ nick translation method and the in situ TUNEL method: Nick translation involves labeling DNA strand breaks with modified …

Oct 15, 2010 · The TdT-mediated dUTP-biotin nick end labeling (TUNEL) assay exploits this biochemical hallmark by labeling the exposed termini of DNA, thereby enabling visualization of …